Senior Research Associate, Biologics - Triveni Bio : Job Details

ABOUT TRIVENI

Triveni Bio is breaking new ground in the identification of novel disease targets – working at the convergence of human genetics, best-in-class antibody design, and precision medicine. We are pioneering a Mendelian genetics-informed precision medicine approach to develop functional antibodies for the treatment of immunological and inflammatory (I&I) disorders. Our lead antibody program (TRIV-509) targets kallikreins 5 and 7 (KLK5/7) to directly impact skin barrier function, inflammation, and itch – providing a meaningful and much needed potential treatment option for patients with atopic dermatitis and other barrier disorders.

SUMMARY

Triveni Bio is in search of a motivated Senior Research Associate to play play an important role in advancing the organization's biologics research and development activities. This position will be focused on the expression, purification, and characterization of monoclonal and bispecific antibodies and antigens using mammalian cell systems, ensuring the production of high-quality proteins. On a day-to-day basis, you will operate and maintain AKTA chromatography systems, employing multiple techniques such as affinity and size exclusion chromatography for purification. In addition to hands-on expression and purification, you will help manage external protein expression projects with CROs, ensuring timely delivery and quality of externally produced proteins. The role also involves conducting rigorous bioanalytical assays, including Biacore, analytical SEC, and ELISA/MSD, to characterize proteins and evaluate critical quality attributes. Lastly, this role supports preclinical activities by preparing protein samples for shipment to external collaborators and CROs and maintaining a comprehensive record of proteins in our internal inventory.

PRIMARY RESPONSIBILITIES

• Design, optimize, and implement experimental workflows for recombinant protein expression, primarily in mammalian and bacterial cell lines.
• Maintain mammalian cell cultures for internal protein expression.
• Purify proteins using various techniques, including affinity chromatography and size-exclusion chromatography (SEC).
• Characterize protein quality and behavior in solution using analytical methods such as SEC and SDS-PAGE.
• Troubleshoot issues in protein expression, purification, and quality control workflows.
• Manage and maintain the FPLC system, ensuring reliable operation and optimal performance to support protein purification workflows.
• Analyze and interpret experimental data, prepare reports, and present findings to internal teams

EXPERIENCE & QUALIFICATIONS

• Bachelor's or Master's degree in Biochemistry, Molecular Biology, or a related field with 2+ years of relevant experience in protein expression, purification and characterization.
• Experience with monoclonal and bispecific antibodies is strongly preferred.
• Hands-on experience with AKTA chromatography systems and proficiency in standard purification techniques such as affinity and SEC.
• Familiarity with bioanalytical techniques, including Biacore, analytical SEC, and ELISA/MSD.
• Proficiency in data analysis/visualization software like GraphPad Prism and bioinformatics software for sequence analysis.
• Other duties as assigned